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Anmol Seelan

The joy of savoring healthy food

It is a hot, humid day. The sun is shinning bright, the leaves of the trees are going dry, and you can see the effects of mirages everywhere. At this very moment, the shopkeeper hands you a cold glass of coca cola. Eager to taste this cold delight on a […]

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Inoculation development for production of recombinant microbial products

Recombinant DNA technology:Recombinant DNA technology introduces foreign genes into plants, animals and microorganisms in order to express new characteristics. This technique has been used in production of livestock, food products, biopharmaceuticals, antibiotics, and industrial chemicals. Recombinant microorganisms are also used for small scale and large-scale fermentations.Inoculum development:An inoculum can be […]

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Automated gel electrophoresis: other methods

Isoelectric focusing: For separations according to differences in isoelectric point (pI), PhastGel IEF media provide stable, linear pH gradients (3–9, 4–6.5 and 5–8) with uniform conductivity across broad and narrow pH ranges. PhastGel IEF media withstand field strengths >500 V/cm for high resolution separations. PhastGel Dry IEF is supplied precast […]

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Automated Gel Electrophoresis System: SDS- Page.

Several analyses are frequently required to monitor and document the stages of a biomolecule purification process. Electrophoresis is typically used to attest the identity, charge, heterogenicity, consistency and/or purity of products. This powerful analytical technique can also give relevant information about the initial and intermediary materials. The initial or starting […]

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Automated Gel Electrophoresis System

Electrophoresis is a standard technique used for various purposes worldwide for DNA, RNA, and protein analysis. It has been known for nearly a century and various techniques have been developed over decades. The basic principle is based on the separation of proteins through application of an electric field across the […]

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Cell cycle: an overview

Cell cycle is the name by which two new cells are replicated and produced. The cell cycle contains several phases known as G1, S, G2, and M. G1 is the stage the cell is about to split. It next progresses to the S phase, which duplicates the whole DNA in […]

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Photocycle of Bacteriorhodopsin

After the absorption of a photon by retinal, this molecule transmits the stimulation to the whole protein. The isomerization of the retinal after the stimulus, besides the contacts with the residues of the RBP, transduces the signal, through the different helices, from the middle of the protein to the cytoplasmic […]

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Bacteriorhodopsin

In a cell, be it prokaryotic or eukaryotic, there are plenty of functions to perform. Most of which are carried out by proteins. Depending on the location, proteins can be soluble or transmembrane or amphitropic. The functions of transmembrane proteins range from signal transduction, ion channels, energy transduction, structural role […]

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Major tools for molecular disease diagnosis

Many diseases are linked to genetic and molecular diseases which are quite detrimental to the health of infected individuals who might even lead a healthy live, but due to their genetic defects, suffer from abnormalities of varying impacts. Genetic diseases are difficult to cure because scientists are faced with the […]

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Molecular Diagnosis of Genetic Diseases

An individual’s genetic composition plays an important role in susceptibility to all diseases. In other words, all diseases have a genetic component to them. But much more needs to be learnt in terms of the degree to which these genes contribute to diseases. Molecular tests can be employed to confirm […]

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Researchers find rare mineral inside the teeth of Chiton

Chitons of the Polyplacophora class are flattened, symmetric to the bilateral. These species exist all across the world, from frigid to tropical waters. Their teeth are one of nature’s most difficult to break materials. They have to be attached to a tongue-like and soft radula, that scraps algae and other food […]

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Capillary gel electrophoresis and applications.

Electrophoresis is a separation method in which charged particles migrate under the influence of an electrical field. They migrate under the influence of electric field at various speeds, depending on their charge to mass ratio. The use of agarose gels in the electrophoresis and use of monolithic column was pioneered […]

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Industrial production of tetracycline antibiotics.

Since the discovery of tetracycline in 1940s, tetracycline has been a very important component in the treatment of various bacterial infections. Tetracycline belongs to the family of antibiotics that inhibits the protein synthesis. Tetracycline prevents the attachment of aminoacyl-tRNA to the ribosomal acceptor site. Tetracycline is a broad-spectrum antibiotic which […]

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Application of FISH

Fluorescence in situ hybridization (FISH) was introduced almost 30 years ago, it was a beginning of a new era in the study of chromosome structure and function. FISH is a very straightforward technique. FISH is a technique in which DNA probes are hybridized to its complementary sequence on the chromosome […]

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Termite-Bacterial association

What is Termite-Bacterial association?? Lower termites are one of the best studied systems in insects. Their ability to feed on a nitrogen poor and wood-based diet with help from symbiotic microbes has been under investigation for almost a century. A microbial consortium living in the guts of lower termites is […]

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High energy molecules

What are high energy molecules??? The high-energy molecules include all the nucleoside triphosphoric and diphosphoric acids, the pyrophosphoric and poly-phosphoric acids, phosphocreatine, phosphopyruvic acid, diphosphoglyceric acid, acetyl coenzyme A, succinyl coenzyme A and the aminoacyl derivatives of adenylic and ribonucleic acids. Introduction: How Cells Obtain Energy from Food: As we […]

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Microbial Biofilms and Mats

What are Microbial Biofilms and Mats?? Biofilms and microbial mats can be defined as surface associated to layers of microbial cells embedded in extracellular polymeric substance. Biofilms cover solid surfaces, while mats cover sediments. A microbial mat is a multi layered sheet of microorganisms mainly consisting of bacteria and archaea. […]

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Fugu: The forbidden fish

Everyone knows that its human nature to want a thing that is forbidden to them. This is what drives many people in Japan and even foreigners who visit Japan, to try the infamous fugu fish. Found in the Pacific Ocean, this fish is a pufferfish and looks rather innocuous, in […]

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IMMUNE DYSFUNCTION AND ITS CONSEQUENCES

 The evolution of immune system. It can be defined as a complex evolutionary unit and it can be said that the immune system of primitive organisms is also primitive. The immune system involves numerous elements, which have undergone many evolutionary changes, some which are neutral, some are selected, to form […]

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